Identification of differentially expressed biliary proteins induced by cholangiocarcinoma using 2D-DIGE
10.3760/cma.j.issn.1007-8118.2011.03.016
- VernacularTitle:荧光差异凝胶电泳筛选胆管癌胆汁差异表达蛋白的研究
- Author:
Bo CHEN
;
Shengquan ZOU
;
Jiangfeng QIU
;
Jianchun CAI
;
Lin XU
;
Peiren WU
;
Ming HONG
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms;
Bile;
Fluorescence two-dimensional difference gel electrophoresis
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(3):231-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the probability of identification of differential expression of biliary proteins induced by cholangiocarcinoma using 2D-DIGE. Methods Bile was obtained from 12patients with obstructive jaundice (including 6 cases of cholangiocarcinoma and 6 of cholelithiasis).Each sample was labeled with three different CyDyes (y3,Cy5,Cy2) including one internal standard,pooled from all the samples, and separated with 2-D DIGE in triplicate experiments. MALDI-TOF-MS and bioinformatics were adopted to identify and elucidate the significance of differentially expressed proteins in bile induced by cholangiocarcinoma. Results 55 matched protein spots differences in abundance were detected with statistical variance of two groups(Average Volum Ratio ≥1.5, t-test, P＜0. 05). Among these proteins, 13 PMF were obtained by MALDI-TOF-MS analysis. Eight proteins were identified by searching a protein database. Conclusion The differentially displayed proteomes between the pathological bile obtained from benign and malignant obstructive jaundice indicates the potential application of 2D-DIGE to identify the biomarker of cholangiocarcinoma.
