Effects of COL8A1 siRNA on the proliferation, invasion and drug sensitivity to D-limonence treatment in hepatocarcinoma cells
10.3760/cma.j.issn.1007-8118.2011.03.018
- VernacularTitle:COL8A1siRNA对肝癌细胞体外增殖、侵袭能力及右旋柠烯药敏性的影响
- Author:
Yongfu ZHAO
;
Li JIA
;
Zhenhai MA
;
Yongji LIU
- Publication Type:Journal Article
- Keywords:
COL8A1;
RNA interferences Hepatocarcinoma;
D-limonence
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(3):239-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible effects of COL8A1 on the proliferation, invasion and drug sensitivity of murine hepatocarcinoma cell line Hca-F, we used an RNA interference (RNAi) approach to silence COL8A1 expression. Methods The expression levels of COL8A1 in HcaF/siRNA cells were assessed by RT-PCR and Western blot. The inhibitory effect of RNAi on Hca-F cell invasion in vitro was demonstrated by ECM invasion assay. The in vitro proliferative ability and drug sensitivity of COL8A1-deficient cells were determined by MTT assay. Results The expression of COL8A1 was significantly reduced in COL8A1/siRNA cells after 30h transfection, compared with both the RNAi control and the Hca-F cells. The reduced COL8A1 expression also attenuated the proliferative, invasive ability, as well as increased drug sensitivity of Hca-F/siRNA cells. Conclusion Our current results indicate that the expression of COL8A1 functionally mediates tumor cell proliferation, invasion, and drug sensitivity, and is a potential target for therapeutic anti-cancer drugs.
