Oncolytic adenovirus vector expressing IL-24 gene suppresses hepatocellular carcinoma in vitro
10.3760/cma.j.issn.1007-8118.2011.03.023
- VernacularTitle:表达IL-24基因的溶瘤腺病毒对肝癌细胞的抑制作用
- Author:
Yi CHEN
;
Dan HAN
;
Binbin LIU
;
Min LIANG
;
Ruixia SUN
;
Zhenggang REN
;
Yanhong WANG
;
Shenglong YE
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Metastasis;
IL-24;
Gene therapy
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(3):257-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the selective oncolytic role and antitumor action of a novel recombinant adenovirus containing E1A and IL-24 on hepatocellular carcinoma cell(HCC). Methods The recombinant adenovirus expressing IL-24 (Ad. HS4. AFP. E1A/IL-24) was constructed by using modified human alpha-fetoprotein (HS4-AFP) promoter to drive adenovirus E1A gene and II-24 gene.Cell Counting Kit-8 were performed to test the selective cytotoxicity of the virus in hepatocellular carcinoma cell lines SMMC-7721, Hep3B, MHCC97-H and hepatocyte cell line L02 . The mRNA and protein expression of IL-24 gene were detected by RT-PCR and western blot. Cell growth curves and Annexin V/PI assay were used to study cell proliferation and apoptosis of MHCC97-H. The anti-metastatic effects of the recombinant adenovirus were evaluated in cell adhesion, migration, and cell motion. Matrix metalloproteinase-2 (MMP-2) expression was examined by RT-PCR and zymography.Results Selective replications of Ad. HS4. AFP. E1A/IL-24 adenovirus were observed in over expression AFP cell line MHCC97-H, a highly metastatic potential HCC cell line but not in hepatocyte cell line L02. The mRNA and protein of IL-24 were also over expressed in MHCC97-H. This recombinant adenovirus also showed the significant oncolytic action on MHCC97-H but not on L02 (P＜0. 05). Besides, the recombinant adenovirus significantly inhibited MHCC97-H metastatic potential such as cell adhesion, migration and invasion as well(P＜0.01). Conclusion The selective oncolytic adenovirus expressing E1A and II-24 has a selective antitumor effect and play an inhibitory role in metastasis of HCC.
