Effects of splencectomy on the expression of PDGF-B in the live and the serum level of PDGF-BB of the rats with hepatic fibrosis
10.3760/cma.j.issn.1007-8118.2011.04.012
- VernacularTitle:脾切除对肝纤维化大鼠肝脏PDGF-B表达及血清PDGF-BB水平的影响
- Author:
Hu ZENG
;
Wanpin NIE
;
Haining LIU
- Publication Type:Journal Article
- Keywords:
Platelet-derived growth factor;
Hepatic fibrosis;
Splenectomy;
Immunohistochemistry
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(4):305-308
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of splenectomy on hepatic fibrosis and on the expression of PDGF-B in the liver and PDGF-BB in the serum of rats with hepatic fibrosis. MethodsBy hypodermic injection CCl4, we established 65 rat models with hepatic fibrosis, splenectomies were performed in the three groups at different phases: before hypodermic injection CCl4 (A group), five weeks after hypodermic injection CCl4 (B group), and ten weeks hypodermic injection CCl4 (C group). The control groups were established at the same time, with samples of the livers and serum of the rats taken in different phases. The expressions of PDGF in the liver were detected by immunohistochemistry technique and the degree of hepatic fibrosis was detected by HE staining. The serum levels of PDGF-BB were analyzed by ELISA technique. ResultsAbsorbance values of PDGF-B in the experimental group were significantly lower than the control groups (P＜0. 05). Serum levels of PDGF-BB of the rats after splenectomy were significantly lower than those in the control groups (P＜0.05). HE and Masson's staining showed that the progression of Hepatic fibrosis was slow in the A group. Hepatic pathologic state was significantly relieved in the B group and the inflammation and fibrosis was relieved in the C group. Conclusion Earlier period splenectomy could delay the proceeding of experimental hepatic fibrosis. After splenectomy the decline in the level of PDGF may be one of the mechanisms causing the delay.
