Effect of peroxisome proliferator activated receptor γ agonist on the proliferation and differentiation of rat osteoblasts in vitro
10.3760/cma.j.issn.0254-9026.2011.03.019
- VernacularTitle:过氧化物酶体增殖物活化受体激动剂对大鼠成骨细胞增殖分化的影响
- Author:
Yan WU
;
Qin XIA
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator-activated receptor;
Osteoblasts;
Transforming growth factor betal;
Alkaline phosphatase
- From:
Chinese Journal of Geriatrics
2011;30(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of rosiglitazone (RSG), the agonist of peroxisome proliferator activated receptor γ (PPARγ), on the proliferation and differentiation of rat osteoblasts and the related mechanisms. Methods The identification of rat primary osteoblasts was performed by alkaline phosphatase (ALP) staining and mineralized nodules. The 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay and p-nitrophenyl phosphate (PNPP) assay were used to observe the effects of different concentrations of RSG on proliferation and differentiation of the osteoblasts. The reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of connective tissue growth factor (CTGF) mRNA. The effects of the different concentrations (0,1,2,5,10 and 20 μmol/L) of RSG on TGF-β1-induced CTGF mRNA expression in osteoblasts were detected. Results (1)Different concentrations of RSG could not change the proliferation of osteoblasts (P＞0. 05). (2)Compared with control group, all different concentrations of RSG could suppress ALP activity in osteoblasts (P＜0. 01 ). (3) RSG suppressed the osteoblats CTGF mRNA expression induced by TGF-β1 in a dose-dependent manner (P＜0. 01). Conclusions In vitro, RSG can inhibit the TGF-β1 induced rat osteoblasts CTGF mRNA expression. RSG may play a potential role in preventing the differentiation of the rat osteoblasts.
