Study on enzyme-linked immunosorbent assay for detecting thrombospondin-1 and its diagnostic value for prostatic carcinoma
10.3760/cma.j.issn.0254-9026.2011.04.012
- VernacularTitle:酶联免疫吸附法测定血小板反应素1及其对前列腺癌诊断价值的研究
- Author:
Yaoguang ZHANG
;
Jianye WANG
;
Ruomei QI
;
Liqing ZHANG
;
Ben WAN
;
Dong WEI
;
Shengcai ZHU
;
Meiyi HE
;
Renshe CHEN
;
Pinling ZENG
- Publication Type:Journal Article
- Keywords:
Hyperplasia of prostate;
Prostate neoplasms;
Blood platelets;
Reagins
- From:
Chinese Journal of Geriatrics
2011;30(4):305-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use enzyme-linked immunosorbent assay (ELISA) for measuring thrombospondin-1 (TSP-1),and to analyze its diagnostic value for prostatic carcinoma.Methods The possible difficulties and the way to solve the difficulties with ELISA spot were explored first.Three agents which could segregate idio-antigen and one technique which could depurate proteinum were designed and developed.The non- idio- proteinum cross reaction problems were solved and the routine method to measure TSP-1 with ELISA was set up successfully.The serum TSP-1 was measured in 14 patients with benign prostatic hyperplasia (BPH) and 18 patients with prostatic carcinoma.Results The TSP-1 values were (73.77±12.72)% and (121.86±-19.47)% in prostatic carcinoma group and benign prostatic hyperplasia group,respectively (t= 8.44,P＜0.01).The diagnostic sensitivity and specificity of TSP-1 and prostate specific antigen (PSA) for prostatic cancer were 92.7%,88.9% and 85.7%,66.7%,respectively (P＜0.01).The area under the receiver operating characteristic curve (ROC) of TSP-1 and PSA were 0.9663 and 0.7421 (P＜0.05).Conclusions The determination of TSP-1 with ELISA is feasible.TSP-1 is an ideal diagnostic parameter for prostatic carcinoma and it may distinguish BPH from malignant prostatic disease more exactly than PSA.
