Clinical significance of extracellular matrix and hepatic ultramicrostructural changes in patients with mild chronic hepatitis B
10.3760/cma.j.issn.1674-2397.2011.01.006
- VernacularTitle:轻度慢性乙型肝炎患者肝脏细胞外基质和超微结构改变与临床相关性分析
- Author:
Wei LIN
;
Chenwei PAN
;
Lu ZHUGE
;
Yi ZHENG
;
Guangyao ZHOU
;
Zhouxi FANG
;
Ximing Lü
;
Linxiang JIN
- Publication Type:Journal Article
- Keywords:
Chronic hepatitis B;
Extracellular matrix;
Ultramicrostructure;
Hepatocytes
- From:
Chinese Journal of Clinical Infectious Diseases
2011;04(1):29-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlations of extracellular matrix and hepatic ultramicrostructural changes with clinical manifestations in patients with mild chronic hepatitis B (CHB).Methods Patients with chronic HBV infections were enrolled and were divided into mild CHB group (n=66) and HBV carrier group (n=10).Serum samples were collected from patients, and serum HBV markers, HBV DNA load and liver fibrosis indexes were measured.All subjects received liver biopsy, and the tissue samples were observed by light microscope and electron microscope.T test and χ2 test were performed for measurement data and enumeration data, respectively.Spearman test was used for ranked data.Results The differences on ALT and AST levels between mild CHB group and HBV carrier group were significant (t=12.42, 7.06, P＜0.05), but there was no significant difference on HBV DNA load between two groups (t=0.24, P ＞ 0.05).Serum liver fibrosis indexes (hyaluronic acid, type Ⅲ collagen,type Ⅳ collagen and laminin protein) in mild CHB group were not significantly higher than those in HBV carrier group (t=0.45, 0.95, 0.76 and 1.21, P ＞0.05).In mild CHB group, there were 33 patients with ≥G2 and ≥S2, but in HBV carrier group were only 2 patients (χ2=4.17, P ＜ 0.05).Seventeen patients in mild CHB group were with S3-4, while that was not observed in HBV carrier group (χ2=4.75, P ＜0.05).In mild CHB group, hepatic ultramicrostrutural changes on fat storing cell, collagen protein and portal area were correlated with fibrosis grades, and the correlation coefficients were 0.351, 0.675 and 0.301, respectively (P=0.004, 0.000 and 0.014).Conclusion Electron microscope is of higher sensitivity than light microscope in observing hepatic ultramicrostructural changes, which is effective in evaluating the severity of mild CHB.
