Extracellular and intracellular IFNγ and IL-4 in evaluating Th1/Th2 responses in children with chronic hepatitis B
10.3760/cma.j.issn.1674-2397.2011.01.007
- VernacularTitle:细胞内外IFNγ和IL-4在评估儿童慢性乙型肝炎Th1/Th2应答中的价值
- Author:
Yi CHEN
;
Donghui SU
;
Chen PAN
;
Zuxiong HUANG
- Publication Type:Journal Article
- Keywords:
Chronic Hepatitis B;
Child;
Interferon γ;
Interleukin-4;
Flow cytometry
- From:
Chinese Journal of Clinical Infectious Diseases
2011;04(1):33-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate extracellular and intracellular IFNγ and IL-4 levels in assessing Th1/Th2 balance in children with chronic hepatitis B (CHB) and asymptomatic carriers.Methods Fiftyfour hospitalized children including 23 CHB patients and 31 asymptomatic carriers were collected from May 2007 to February 2009.Thirty-four healthy children were served as control.Serum IFNγ and IL-4 levels were measured by enzyme-linked immunosorbent assay (ELISA) and intracellular IFNγand IL-4 levels were detected by flow cytometer.Analysis of variance (for homogenous variance) and Kruskal-Wallis test (for non-homogenous variance) were performed.Results The differences on extracellular IFNγ, IL-4 and Th1/Th2 among CHB, asymptomatic carriers and control groups were not statistically significant (F=0.342, 0.020 and 0.507, P ＞ 0.05); while the intracellular IFNγlevels were (7.68 ± 4.62), (11.71 ±4.36) and (13.61 ±6.71) μg/mL, and Th1/Th2 ratios were 0.96 ±0.30, 1.67 ±0.76 and 2.11 ± 1.12in three groups respectively (F=0.255 and 0.140, P ＜ 0.05 or ＜ 0.01).The differences in intracellular IL-4 levels among three groups were not significant (F=0.425, P ＞ 0.05).Conclusions Cytokine balance is affected in CHB children and asymptomatic carriers, and flow cytometry analysis is considered as a better method in evaluating the status of Th1/Th2 balance.
