Assays for detection of human papillomavirus infection in diagnosis of high grade cervical lesions
10.3760/cma.j.issn.1674-2397.2011.02.006
- VernacularTitle:不同人乳头状瘤病毒检测方法对宫颈高度病变的诊断价值
- Author:
Hualin XU
;
Yingyan LU
;
Hongmei LIN
;
Pingping TAO
- Publication Type:Journal Article
- Keywords:
Papillomavirus infections;
Uterine cervical neoplasms;
Hybrid capture Ⅱ;
Polymerase chain reaction
- From:
Chinese Journal of Clinical Infectious Diseases
2011;04(2):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of different assays for detection of human papillomavirus(HPV)in diagnosis of high grade cervical lesions.Methods Two hundred subjects with abnormal thinprep liquid-based cytology test(TCT)Resultswere selected for HPV DNA detection by hybrid capture 2(HC-Ⅱ) and Methodsbased on PCR including flow-through hybridization and gene chip (HybriMax),real-time fluorescent quantitative PCR(FQ-PCR)and flow fluorescent hybridization assay.Cytopathological Resultswere used as gold standards to evaluate the test performance of the above assays for diagnosing cervical intraepithelial neoplasia(CIN)≥Ⅱ. SPSS 13.0 software was used for statistical analysis.Results HPV DNA positive rates of 200 samples by HybriMax,FQ-PCR,flow fluorescent hybridization assay and HC-Ⅱ were 72.5%(145/200),71.5%(143/200),70.0%(140/200)and 69.0%(138/200),respectively,and the differences were not statistically si(g)nificant(x2 =0.252,0134,0.012 and 0.027,P ＞ 0.05).The sensitivity,Youden index and negative predictive value of the above assays were statistically different(x2 =7.923,7.819 and 8.108,P ＜0.05).Conclusion HC-Ⅱ is superior to PCR Methodsin diagnosis of CIN Ⅱ and above.
