Effect of thyroid hormones on the expression of thyroid hormone receptor isoforms in human osteogenic sarcoma cell line MG-63
10.3760/cma.j.issn.1000-6699.2011.03.015
- VernacularTitle:甲状腺激素对成骨样MG-63细胞甲状腺激素受体不同亚型表达的影响
- Author:
Yuhong YANG
;
Aijun ZUO
;
Zhifeng ZHU
;
Xueqin ZHAO
;
Gang GUO
;
Jingyu ZHANG
- Publication Type:Journal Article
- Keywords:
Thyroid hormones;
Thyroid hormone receptors;
Human osteoblast-like cell line MG-63;
Gene expression
- From:
Chinese Journal of Endocrinology and Metabolism
2011;27(3):242-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of thyroid hormone receptor isoforms (TR α1, α2, β1, and β2) in human osteoblast-like cell line MG-63 at the mRNA level and the effect of thyroid hormone (T3 or T4 ) on the expression. Methods Realtime quantitative PCR was performed. Results The expression of TRα1 mRNA was the highest, that was 10. 70± 0.45, TRβ1 was 5.75 ± 0. 10, TRβ2 was 3.34 ± 0. 08, and TRα2 was very low, only (3.66 ±0. 59) × 10-2. Only the expression of TRαl and TRα2 mRNA was down regulated significantly by the treatment of 10-10 ～ 10-6 mol/L T3, and there was a negative correlation between the expression of TRα1 or TRα2 mRNA and the concentration of T3. Conclusion TRα1 plays a primary role in mediating the effects of thyroid hormones in skeletal development.
