Effect of calcitonin on analgesia and expression of calcitonin receptor in periaqueductal gray
10.3760/cma.j.issn.1000-6699.2011.02.005
- VernacularTitle:降钙素的镇痛作用及其对中脑导水管周围灰质中降钙素受体表达的影响
- Author:
Shufa LI
;
Fei XIANG
;
Taowei ZHANG
;
Chao JIANG
;
Jianyong ZHAO
;
Min ZHANG
;
Yuan LIANG
;
Bing CHEN
- Publication Type:Journal Article
- Keywords:
Calcitonin;
Analgesia;
Chronic constriction injury;
Periaqueductal gray;
Calcitonin receptor
- From:
Chinese Journal of Endocrinology and Metabolism
2011;27(2):113-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the analgesic effect of salmon calcitonin(sCT)and its effect on expression of calcitonin receptor(CT-R)in periaqueductal gray(PAG). Methods Rat models of neuropathic pain were prepared by chronic constriction injury(CCI). Thermal withdrawal latency(TWL)and mechanical nociceptive threshold(MNT)were measured using hot plate test and yon Frey monofilaments test. The distribution of CT-R in PAG was detected by immunohistochemical method. CT-R protein was quantitatively determined by western blotting. Fourty male SD rats were randomized into 5 groups: normal group, sham-CCI group, CCI group, CCI plussubcutaneous sCT group, and CCI plus microinjection of sCT into PAG group. Results TWL, MNT, andexpression of CT-R in PAG showed no difference between normal group and sham-CCI group(P＞0. 05). TWL and MNT in CCI group were significantly lower than those in normal group(P＜0.05), and expression of CT-R in CCI group was significantly higher than that in normal group(P＜0.05). TWL, MNT and expression of CT-R in CCI rats increased significantly after sCT therapy(P＜0. 05), and the effect was more marked in PAG injection group than subcutaneous injection group(P＜0.05). Conclusions sCT raises the pain threshold and increase the expression of CT-R in PAG of CCI rats, while PAG injection showed more marked effect than subcutaneous injection.
