The thyroid hornone receptor in human osteogenic sarcoma cell line MG-63 cell-T3 binding studies with nuclear extracts

VernacularTitle:成骨样细胞MG-63中的甲状腺激素受体Ⅰ——细胞核可溶性甲状腺激素受体与激素(T3)结合的动力学
Author: Yuhong YANG ; Aijun ZUO ; Zhifeng ZHU ; Xueqin ZHAO ; Gang GUO ; Jingyu ZHANG
Publication Type:Journal Article
Keywords: Thyroid hormone receptors; Human osteoblast-like cell line MG-63; Scatchard analysis
From: Chinese Journal of Endocrinology and Metabolism 2011;27(2):125-127
CountryChina
Language:Chinese
Abstract: To establish a method for radioligand binding assay of thyroid hormone receptors(TR)in human osteoblast-like osteosarcoma cell line MG-63 and to estimate the kinetic parameters of putative receptors. The MG-63cell was cultured in Ham's F12, the soluble TR was prepared from the intact nuclear extracts. The binding properties between TR and T3 were performed by using the traditional Scatchard analysis. The apparent Ka of TR in MG-63 is 7.68× 109 L/mol, and MBC(111. 25+ 10.77)fmol/mg protein. The study indicated that MG-63 cells possessed high affinity and limited-capacity of TR in its nuclear extracts. This may serve as the starting and basic work about TR in bone cell.