Effects of cytokines on expression of angiotensin Ⅱ type 1 receptors in vascular smooth muscle cells in rats
10.3760/cma.j.issn.0254-1416.2011.01.031
- VernacularTitle:细胞因子对大鼠血管平滑肌细胞血管紧张素Ⅱ1型受体表达的影响
- Author:
Jianrong GUO
;
Lijun LIAO
;
Donglin JIA
;
Jun YU
;
Wei GUO
;
Huachun SHEN
- Publication Type:Journal Article
- Keywords:
Cytokines;
Receptor,angiotensin,type 1;
Muscle,smooth,vascular
- From:
Chinese Journal of Anesthesiology
2011;31(1):105-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of cytokines on the expression of angiotensin Ⅱ type 1 receptor (AT1R) in vascular smooth muscle cells (VSMCs) in rats. Methods Primary cultured VSMCs from SD rat thoracic aorta were cultured in serum-free DMEM for 24 h, and then in DMEM supplemented with 10% fetal bovine serum for another 12 h. The cultured VSMCs were randomly divided into 5 groups (n =6 each): control group (group C); 10% cytokine group (group L); 50% cytokine group (group N); 100% cytokine group (group H) and L-arginine methy ester (L-NAME), an inhibitor of nitric oxide synthase) group. In group C, the cellswere cultured continuously for 12 h. In L, N and H groups, 10%, 50% and 100% cytokines (IL-1β 50 ng/ml +TNF-α 100 ng/ml + IFN-γ 500 ng/ml) were added to the culture medium respectively and the cells were then incubated for 12 h. In group L-NAME, 100% cytokines + L-NAME 5 mmol/L were added to the culture medium and the cells were then incubated for 12 h. The expression of AT1R mPNA and protin was determined by RT-PCR and Western blot respectively.Results Cytokines down-regulated AT1R mRNA and protein expression in a concentration-dependent manner (P ＜ 0.05 or 0.01). L-NAME reversed cytokines-induced changes in AT1R mRNA and protein expression ( P ＜ 0.01). Conclusion Cytokines can down-regulate the expression of AT1R in rat VSMCs and the mechanism is related to the NO synthesis.
