Effects of dexmedetomidine on expression of Toll-like receptor 4 mRNA in rat peripheral blood monocytes exposed to lipopolysaccharide
10.3760/cma.j.issn.0254-1416.2011.01.034
- VernacularTitle:右美托咪啶对脂多糖诱导大鼠外周血单核细胞Toll样受体4mRNA表达的影响
- Author:
Donglai YAN
;
Yonghao YU
;
Hongwei LIU
;
Jing TIAN
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Lipopolysaccharides;
Toll-like receptor 4;
Monocytes
- From:
Chinese Journal of Anesthesiology
2011;31(1):115-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of dexmedetomidine on the expression of Toll-like receptor 4 (TLR4) mRNA in rat peripheral blood monocytes exposed to lipopolysaccharide ( LPS ). Methods Peripheral blood monocytes isolated from male Wistar rats were seeded in 24-well plate in RPMI 1640 liquid culture medium in CO2 incubator at 37 ℃ and 5% CO2 for 2 h, and were randomly divided into 5 groups ( n = 8 each): group A negative control; group B was exposed to LPS 1 μg/ml and C, D and E groups were exposed to LPS 1 μg/ml + dexmetomidine 0.5, 5.0 and 50.0 ng/ml respectively. The monocytes were then incubated for 24 h. The concentrations of TNF-α, IL-1β and IL-6 in the supernatant of the cultured monocytes were detected by ELISA. The expression of TLR4 mRNA in the monocytes was detected by RT-PCR.Results Exposure to LPS significantly increased the expression of TLR4 mRNA and the concentrations of TNF-α, IL-1β and IL -6 in group B as compared with group A ( P ＜ 0.01 ). Dexmedetomidine attenuated the LPS-induced increase in the expression of TLR 4 mRNA and the concentrations of TNF-α, IL-1β and IL-6 in a dose-dependent manner ( P ＜0.05or 0.01 ). Conclusion Dexmedetomidine can inhibit the synthesis of TLR4 and inhibit the secretion and dilivery of TNF-α, IL-1β and IL-6 by down-regulating the gene expression of TLR4 in rat peripheral blood monocytes exposed to LPS.
