Effects of morphine on PTEN expression and NF-κB activity in human gastric carcinoma cell line MGC-803

VernacularTitle:吗啡对人胃癌MGC-803细胞磷酸酶基因表达和NF-κB活性的影响
Author: Yi QIN ; Jing CHEN ; Zhiling XIAO ; Yubo XIE ; Qiang XIAO
Publication Type:Journal Article
Keywords: Morphine; Stomach neoplasms; Apoptosis; PTEN phosphohydrolase; NF-kappa B
From: Chinese Journal of Anesthesiology 2010;30(12):1446-1448
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of morphine on PTEN expression and NF-κB activity in human gastric carcinoma cell line MGC-803.Methods The human gastric cancer cell line MGC-803 was purchased from Cell Biology Research Institute,Chinese Academy of Sciences,and cultured in DMEM liquid culture medium.The cells were randomly divided into 2 groups(n = 6 each): control group and morphine group.The cells was exposed to 0.1 μmol/L morphine in morphine group.The apoptosis was assessed by flow cytometry after being incubated with morphine for 24 h.PTEN expression and NF-κB activity were detected using RT-PCR and Western blot.Results The apoptotic rate was significantly increased,PTEN expression was up-regulated and NF-κB activity was significantly decreased in morphine group compared with control group(P ＜ 0.05).Conclusion Morphine can promote the apoptosis in human gastric cancer cells by up-regulating PTEN expression and decreasing NF-κB activity.