Effects of emulsified isoflurane preconditioning on myocardial NF-κB activity during ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2010.12.019
- VernacularTitle:乳化异氟醚预处理对大鼠心肌缺血再灌注时NF-κB活性的影响
- Author:
Chan SHEN
;
Yan RAO
;
Yanlin WANG
;
Zongze ZHANG
;
Chengyao WANG
;
Hui LI
;
Yun WANG
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Myocardial reperfusion injury;
NF-kappa B
- From:
Chinese Journal of Anesthesiology
2010;30(12):1469-1471
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of emulsified isoflurane preconditioning on myocardial NF-κB activity during ischemia-reperfusion(I/R)in rats.Methods Forty-eight healthy male SD rats weighing 230-280 g were randomly divided into 4 groups with 12 animals in each group: sham operation group(group S),I/R group,lipid emulsion + I/R group(group L)and emulsified isoflurane + I/R group(group EI).In group I/R,EI and L,myocardial I/R was produced by occlusion of left coronary anterior descending artery(LAD)for 30 min followed by 120 min reperfusion.In group L,30% lipid emulsion 4 ml·kg-1 ·h-1 was infused intravenously for 30 min before myocardial I/R.In group EI,emulsified isoflurane 4 ml· kg- 1 · h- 1 was infused intravenously for 30 min followed by 15 min washout before myocardial I/R.In group S and I/R,normal saline was given instead.Blood samples were taken from femoral artery at the end of 120 min reperfusion for determination of serum cTnI and IL-6 concentrations and CK-MB activity by ELISA.The rats were then killed and the myocardial tissues were taken for determination of NF-κB activity by Western blot and observation of the ultrastructure by electron microscopy.Results The NF-κB activity,serum cTnI and IL-6 concentrations and CK-MB activity were significantly higher in group I/R,EI and L than in group S(P ＜ 0.05 or 0.01),while lower in group EI than in group I/R(P ＜ 0.05).Microscopic examination showed that emulsified isoflurane significantly attenuated the histopathological changes in group EI.Conclusion Emulsified isoflurane pretreatment can attenuate myocardial I/R injury through decreasing the NF-κB activity and inhibiting inflammatory response in rats.
