Effect of antisense oligonucleotides of telomerase on the growth of RCZ cells derived from renal cell carcinoma in culture
- Author:
Ning XU
;
Zhongwen ZHAO
;
Aiping SHI
;
Chunxi WANG
- Publication Type:Journal Article
- Keywords:
telomerase;
antisense nucleotides;
renal cell carcinoma
- From:
Journal of Jilin University(Medicine Edition)
2000;26(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antisense inhibitor of telomerase on growth of tumor cells.Methods:Phosphorothioate antisense oligonucleotides (PS-ASON) with sequence TAGGGTTAGACAA which can recognize the RNA template region of telomerase and PS-random primer with 13-nucleotides were incubated with RCZ cell line derived from renal cell carcinoma (RCC).The number of cells was counted from 1 to 43 days,doubling time was calculated and inhibiting rate was determined by MTT method.Results ASON group reduced the number of cells (P<0.01), lengthened doubling time (P<0.005),and elevated the inhibiting rate (P<0.01) compared with random primer control and empty control.The efficacy depended on the dose of ASON (P<0.05) and it was of sequence-selective specificity.Conclusion The ASON targeted to the RNA template has an inhibition effect on the growth of RCZ cell and may have potential significance in tumor therapy.
