The effect of brucine on hepatocellular carcinoma cell lines in vitro
10.3760/cma.j.issn.1007-631X.2011.03.016
- VernacularTitle:马钱子碱抗肝细胞癌作用的实验研究
- Author:
Jianmin QIN
;
Xiajun XU
;
Xia SHENG
;
Qi LI
;
Peihao YIN
;
Min ZHANG
;
Lin YANG
;
Zhongqiu SA
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Brucine;
Drug screening assay,antitumor
- From:
Chinese Journal of General Surgery
2011;26(3):219-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of brucine on the growth of a hepatocellular carcinoma cell line in vitro. Methods Brucine was added into a liver cancer cell line of SMMC-7721 in vitro, at drug concentration of brucine from 2. 5 μg/ml to 400 μg/ml. The inhibition rate of cell growth was measured by MTT technique after the cells were cultured for 72 hours. The protein and mRNA expression of PCNA,cyclin D1 and FAS were respectively assayed with Western blotting and fluorescent quantitation RT-PCR techniques at 24, 48, 72 h. Results The inhibition rate of liver cancer cell was near 100% when the brucine concentration was at 320 μg/ml. The protein and mRNA expression of FAS were of no significant difference at 24 h vs 48 h ( seperately F = 2. 547,1. 582, all P ＞ 0. 05 ), and significant difference existed at 24 h vs 72 h( seperately F = 1. 036, 1. 137, all P ＜ 0. 05 ). The protein and mRNA expression of PCNA,Cyclin D1 were of no significant difference between various time period( seperately PCNA F = 3.612,2. 174,3.029;Cyclin D1 F=2.361,2.915,1.976,all P＞0.05). Conclusions Brucine inhibits the growth of liver cancer cells, by inducing increased apoptosis of the cells probably through FAS overexpression.
