Abl interactor 1 knock-down inhibits the in vitro proliferation and migration of NCI-N87 gastric cancer cells
10.3760/cma.j.issn.1007-631X.2011.03.018
- VernacularTitle:敲低Abl相互作用蛋白1的表达抑制胃癌细胞NCI-N87的体外增殖和迁移
- Author:
Mei LI
;
Zhengguo QIAO
;
Xiuying PAN
;
Peiying HE
;
Na HAN
;
Weidong YU
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Cell proliferation;
Cell movement;
Abl-interactor protein 1
- From:
Chinese Journal of General Surgery
2011;26(3):225-228
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of ABI-1 gene knockdown upon the proliferation and migration of human gastric cancer cell NCI-N87 in vitro. MethodsNCI-N87-ABI-I-ShRNA cell model was successfully constructed and validated by Real-time PCR and Western blot. The cellular morphous and skeleton, proliferative and migrative potents, and also AKT expression were compared between NCI-N87-ABI-1-ShRNA and its parents by immunofluorental staining, CCK-8 assay, transwell chamber and Western blotting.ResultsCCK-8 assay showed there was no significant difference in the proliferation rates at different time points between the NCI-N87-Vector and NCI-N87 cells while the proliferation rates at the time points of 36 and 48 hours of the NCI-N87-ABI-1-ShRNA were significantly lower than the NCI-N87( t =2. 85and 4. 166, P ＜ 0. 05 ). Transwell assay showed that migrated cell number were 66 ± 8, 65 ± 8 and 30 ± 4,respectively, and there was significant difference between the NCI-N87-ABI-1-ShRNA and NCI-N87 cells (t =9. 550,P ＜0. 05). Finally, ABI-1- knock-down altered the cellular morphoos and skeleton of 90％NCI-N87 cells and inhibited p-AKT expression.ConclusionABI-1 inhibits proliferation and migration of NCI-N87 cells in vitro probably by PI3K/AKT pathway.
