Dynamic changes of CD4 + CD25 high regulatory T cells in peripheral blood of severe acute pancreatitis patients and its significance
10.3760/cma.j.issn.1007-631X.2010.12.017
- VernacularTitle:重症急性胰腺炎患者外周血CD4+CD25high调节性T细胞的动态变化研究
- Author:
Zhonghou RONG
;
Heshui WU
;
Zhiyong YANG
;
Chunyou WANG
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Systemic inflammatory response syndrome;
T lymphocytes,regulatory
- From:
Chinese Journal of General Surgery
2010;25(12):992-994
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the dynamic changes in CD4 + CD25 high regulatory T cells (Treg), Foxp3, IL-10, IFN-γand IL-4, in peripheral blood of severe acute pancreatitis(SAP) patients and the clinical significance. Methods From August 2008 to June 2009, 42 hospitalized SAP cases were divided by stage according to the course of disease: phase Ⅰ (the period of SIRS) ;phase Ⅱ (the period in which SIRS ends); phase Ⅲ ( the period during which complication related infection developes). Results were compared with those of hospitalized 38 mild acute pancreatitis(MAP) cases during the same period.Treg and Foxp3 were measured by flow cytometry, the levels of IFN-γ、IL-4 and IL-10 were detected by enzyme-linked immunosorbent assay( ELISA), and the relationships between these indicators were analyzed using Pearson correlation analysis test. Results The percentages of Treg and levels of IL-4 increased in phase Ⅰ SAP(Treg: 1.37% ± 1.12% ;IL-4:22.92 ±4. 17) ,soared in phase Ⅱ (Treg:3.12% ± 1.21%;IL-4:35.42 ± 12. 50) ,culminated in phase Ⅲ( Treg:4. 47% ± 1.04%; IL-4:76. 04 ± 14. 58 ); IFN-γ level was the highest in phase Ⅰ SAP(978.57 ±213.29), decreased in phase Ⅱ (571.43 ± 157. 14) ,and the lowest in phase Ⅲ ( 357. 14 ± 150.00 ); The differences were statistically significant between the three periods(P＜0.05). All that in these three periods were higher than that in MAP controls(P ＜0.05).Pearson correlation analysis test showed that quantities of Treg were negatively proportional to the levels of IFN-γ( r = - 0.895, P ＜ 0.01 ), and positively proportional to the levels of IL-4 ( r = 0.813, P ＜ 0.01 ).Conclusions By suppressing IFN-γand promoting IL-4 ,Treg may protect patients against damage resulting from excessive inflammatory response.
