Effects of Apr-1 gene on cell cycle regulation of cholangiocarcinoma QBC939 cell lines
10.3760/cma.j.issn.1007-631X.2011.02.026
- VernacularTitle:Apr-1基因对胆管癌细胞QBC939细胞周期的调节机制研究
- Author:
Jianyong ZHENG
;
Qinlong LI
;
Chunyan DONG
;
Qingchuan ZHAO
;
Jianjun DU
;
Jipeng LI
;
Wei YAN
;
Guangshun YANG
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms;
Cell cycle;
Gene transfer techniques;
Gene expression regulation
- From:
Chinese Journal of General Surgery
2011;26(2):154-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and the mechanism of Apr-1 gene on cholangiocarcinoma QBC939 cell lines proliferation and cell cycle regulation. Methods Apr-1 gene was transfected into QBC939 cells by using liposomes to establish a QBC939 cell model ( QBC939-Apr-1 ) stably expressing Apr-1 gene. Apr-1 mRNA expression and the changes in cell cycle and cell growth of QBC939 cells were analyzed by RT-PCR, flow cytometry ( FCM ) and growth curve before and after transfection. The regulatory effect of Apr-1 gene on the expression of cell cycle-related genes was investigated in QBC939 cells before and after Apr-1 transfection using cell cycle gene microarrays. Results Significant suppression of cell growth was observed with the cell model stably expressing Apr-1 gene. Apr-1 over-expression caused cell arrest from 9% to 13% (P ＜0. 01 ) increase in G2 population. Cell cycle gene microarrays demonstrated that the expression of Skp2 、UBE1 was up-regulated, while the expression of MRE11A 、CKS2 、CDK8 、CDC45 was down-regulated by more than 3 folds. Conclusions Apr-1 gene suppresses QBC939 cell proliferation in vitro, QBC939 cells presented with differences in the expression of cell cycle-related genes after Apr-1 gene transfection.
