Expressions of Notch receptors and target genes in bone marrow CD34+ cells from patients with psoriasis

VernacularTitle:银屑病患者骨髓CD34+细胞Notch受体及其靶基因表达的研究
Author: Ruixia HOU ; Junqin LI ; Ruifeng LIU ; Xinhua LI ; Jing ZHANG ; Guohua YIN ; Kaiming ZHANG
Publication Type:Journal Article
Keywords: Psoriasis; Antigens,CD34; Receptor,Notch; Target gene
From: Chinese Journal of Dermatology 2011;44(3):168-170
CountryChina
Language:Chinese
Abstract: Objective To study the expressions of Notch receptors (Notch1 and 2) and target gene Hes-1 in bone marrow CD34+ cells from patients with psoriasis. Methods CD34+ cells were isolated from the bone marrow of 12 patients with psoriasis and 19 normal human controls. Western blot was conducted to detect the expressions of Notchl, Notch2 and Hes-1 proteins in CD34+ cells, with the house-keeping gene β-actin as an internal reference. Results In contrast with normal controls, a significant increment was observed in the expressions of Notch1 and Hes-1 proteins in psoriatic patients (0.9488 ± 0.3221 vs. 0.6693 ± 0.1465, 0.8579 ±0.3729 vs. 0.5728 ± 0.1787, both P ＜ 0.05). No significant difference was observed in the expression of Notch2 between psoriatic patients and normal controls (0.7568 ± 0.3461 vs. 0.8312 ± 0.2887, P ＞ 0.05).Conclusion The overexpression of Notch1 and Hes-1 may be associated with the low proliferation activity of psoriatic hematopoietic cells.