Differential expression of mRNAs associated with low-dose UVA irradiation-induced adaptation reaction in cultured human melanocytes
10.3760/cma.j.issn.0412-4030.2011.03.010
- VernacularTitle:低剂量UVA诱导的培养人黑素细胞适应性反应中mRNA差异表达研究
- Author:
Zhongrong LIU
;
Huilan YANG
;
Jie LIANG
;
Xuemei LI
- Publication Type:Journal Article
- Keywords:
Ultraviolet rays;
Melanocytes;
Gene expression profiling;
Adaptation reaction
- From:
Chinese Journal of Dermatology
2011;44(3):182-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen for genes associated with low-dose UVA irradiation-induced adaptation reaction in cultured human melanocytes, and to explore the molecular mechanism of the adaptation.Methods Cultured human melanocytes of fifth to tenth passage were divided into two groups, experimental group was subjected to an irradiation with UVA at 7.2 J/cm2 once daily for 4 times and an additional irradiation at a lethal dose of 86.4 J/cm2 6 hours after the above 4-session irradiation, and control group subjected to a single irradiation with UVA at 86.4 J/cm2. A human genome-wide oligonucleotide chip was used to screen for differentially expressed genes between the two groups of cells followed by a functional classification based on international standard. Moreover, a part of these genes were analyzed and identified by real-time fluorescent quantitative PCR. Results In the adaptation reaction induced by low-dose UVA irradiation in cultured melanocytes, there were 129 differentially expressed genes, including 81 up-regulated genes and 48 down-regulated genes. These genes were found to be mainly involved in metabolism, transport, signal transduction, apoptosis, DNA synthesis and repair, and some of them were oncogenes or anti-oncogenes. Real-time PCR confirmed some of the differentially expressed genes. Conclusions The whole genome-wide oligonucleotide chip could screen with high efficiency for differentially expressed genes in low-dose UVA irradiation-induced adaptation reaction in cultured melanocytes.
