A study of tracking the superparamagnetic iron oxide and enhanced green fluorescent protein labeled miniature porcine bone marrow stem cells by in vitro MRI
10.3760/cma.j.issn.0578-1426.2011.04.014
- VernacularTitle:小型猪骨髓间充质干细胞超顺磁氧化铁、增强型绿色荧光蛋白双标记及体外磁共振成像示踪的研究
- Author:
Kuanxiao TANG
;
Yunfeng SHEN
;
Xubin YANG
;
Huimin GU
;
Hejiang DUAN
;
Zhaoxia YAN
;
Jianping WENG
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Diabetes mellitus,type 1;
Magnetic resonance imaging;
Swine,miniature;
Cell tracing
- From:
Chinese Journal of Internal Medicine
2011;50(4):322-327
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To track bone marrow stem cells (BMSCs) labeled by enhanced green fluorescent protein (EGFP) and superparamagnetic iron oxide ( SPIO ) -poly-L-lysine (PLL) compound by MRI in vitro for autotransplantation into pancreas of type 1 diabetes miniature pigs. Methods The BMSCs were isolated by density gradient centrifugation and attachment culture from type 1 diabetes minipigs' bone marrow. Expressional intensity of EGFP in BMSCs transfected lentivirus-EGFP with a multiplicity of infection Different magnetic resonance scanning protocols were carried out on various density BMSCs labeled by different concentration of SPIO in various time-point in vitro. Results When SPIO concentration was 25mg/L (count in Fe3 + ), the positive Fe3+ -labeling rate of BMSCs was 93. 1%. Most of SPIO particles in BMSCs' cytoplasm were observed in secondary lysosomes, but they were not detected in important organelle as cell nucleus. Comparing with gelatin the MRI of BMSCs labeled with SPIO in the condition with 1 ×104/ml cells density and 25 mg/L Fe3+ concentration in vitro, the signal intensity changes (△SI) after BMSCs labeled with SPIO 3 weeks and 6 weeks in TSE T1WI, TSE T2WI and FLASH T2 * WI sequences were 12%, 41%, 63% and 7%, 28%, 46% respectively (P ＜ 0.01 and P ＜ 0.05, respectively).Conclusions The data showed that the porcine BMSCs labeled with SPIO and EGFP could be traced successfully in vitro by MRI in the suitable sequences.
