Functional metabotropic glutamate receptor 1 and 5 expression in podocytes
10.3760/cma.j.issn.1001-7097.2011.02.008
- VernacularTitle:足细胞表达有功能的代谢型谷氨酸受体1和5
- Author:
Leyi GU
;
Xinyue LIANG
;
Lihua WANG
;
Zhaohui NI
;
Yucheng YAN
;
Jiayuan GAO
;
Shan MOU
;
Qin WANG
;
Jiaqi QIAN
- Publication Type:Journal Article
- Keywords:
Podocytes;
Receptors,metabotropic glutamate;
Cyclic AMP response element-binding protein;
Cyclic adenosine monophosphate;
Calcium;
Proteinuria
- From:
Chinese Journal of Nephrology
2011;27(2):100-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of metabotropic glutamate receptor (mGluR) in murine podocytes.Methods Conditional immortalized podocytes were used in the research.RT-PCR was used to estimate the mRNA expression.Western blotting,immunofluorescence staining and immunoelectron microscopy were employed to determine the protein production.EIA,EMSA and Western blotting were used to examine the cAMP generation and cAMP response element-binding protein (CREB) activation.Intracellular calcium was investigated using confocal microscopy.Results mGluR1 and 5 mRNA and protein were expressed in murine brain and podocytes.In glomeruli,most of mGluR1 expression located in podocytes and was expressed in the submembrane space of the podocytes.Podocytes treated with (S)-3,5-dihydroxyphenylglycine (DHPG,an agonist for mGluR1/5) rapidly generated cAMP and activated CREB.(RS)-1-Aminoindan-1,5-dicarboxylic acid (AIDA,a selective antagonist of mGluR1/5) and SQ22536 (an adenylate cyclase inhibitor),but not 2-aminoethoxydiphenyl borate (2-APB an antagonist of canonical transient receptor potential) blocked DHPG-induced cAMP generation and CREB activation.Following DHPG treatment,intracellular calcium level rose and was prevented by pre-treatment with AIDA and 2-APB.DHPG-induced calcium influx was also prevented by incubation with calcium-free medium.Conclusion Podocytes express functional mGluR1 and mGluR5.
