Effect of endoplasmic reticulum stress on phenotypic change of cultured human glomerular mesangial cells induced by high glucose
10.3760/cma.j.issn.1001-7097.2011.02.009
- VernacularTitle:内质网应激在高糖诱导人肾脏系膜细胞表型转化中的作用
- Author:
Yanhong GUO
;
Kun ZHOU
;
Wei QI
;
Wei ZENG
;
Zhifeng LUO
;
Jiao MU
;
Zilin YE
;
Fahuan YUAN
;
Bing FENG
- Publication Type:Journal Article
- Keywords:
Mesangial cells;
Endoplasmic reticulum;
Stress;
Phenotypic change;
4-phenylbutyric acid
- From:
Chinese Journal of Nephrology
2011;27(2):106-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of endoplasmic reticulum stress in phenotypic change of cultured human glomerular mesangial cells induced by high glucose.Methods Cultured human glomeruar mesangial cells were divided into three groups: control group,high glucose group and high glucose+ 4-phenylbutyric acid (4-PBA) group.Cell number of proliferation was assessed by MTT assay.Cell cycle was measured by flow cytometric analysis.Expression of α-SMA was assessed by immunohistochemistry and was observed by laser scanning confocal microscope.Involved mRNA and protein expression were measured by real-time PCR and Western blotting.Results (1)Cell number of proliferation and S transition proportion in high glucose group significantly increased than that in control group (P ＜ 0.05).High glucose could induce α-SMA expression significantly (P＜0.05).4-PBA could significantly inhibit human glomerular mesangial cells proliferation (P＜0.05),S transition arrest (P＜0.05) and expression of α-SMA (P＜0.05) induced by high glucose.(2) Compared with control group,high glucose could significantly increase the expression of glucose-regulated protein78(Grp78 ) mRNA and protein (P＜ 0.05),which could be inhibited by 4-PBA treatment (P＜0.05).(3)High glucose could induce the mRNA and protein expression of TGF-β1 and FN significantly,which could be inhibited by 4-PBA treatment (P＜0.05).Conclusion Endoplasmic reticulum stress plays an important role in phenotypic change of cultured human glomerular mesangial cells induced by high glucose.
