Expression of CXCR6 in mice with transplant rejection following heart transplantation
10.3760/cma.j.issn.0254-1785.2011.04.014
- VernacularTitle:CXC趋化因子受体6在小鼠心脏移植排斥反应中的表达及意义
- Author:
Xiaofeng JIANG
;
Lei ZHU
;
Zheming CUI
;
Dawei GUO
;
Wewyu SUN
;
Lin LIN
;
Xuefan WANG
;
Yufu TANG
;
Jian LIANG
- Publication Type:Journal Article
- Keywords:
Mice;
Heart transplantation;
Graft rejection;
Receptors,chemokine,CXC6
- From:
Chinese Journal of Organ Transplantation
2011;32(4):245-248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CXCR6 in allograft rejection and effect of CXCL16/CXCR6 interaction on allograft survival Methods Intra-abdominal heterotopic heart transplantation was performed using wild type (WT) Balb/c mice (H-2d) (allogeneic) as donors or WT C57BL/6 mice (B6, H-2b) (syngeneic) as donors, and using WT B6 mice as recipients. The intragraft expression of CXCR6 and expression of CXCR6 in CD8+ T cells of the spleens from syngeneic and allogeneic recipients were examined. The allogeneic recipients were further divided into the experimental group (n = 5) and control group (n = 6) randomly. The experiment group and control group were injected with anti-CXCL16 mAb or control mAb respectively until rejection occurred. The cardiac allograft survival in experimental group and control group was evaluated. Results Rejected allografts showed higher expression of CXCR6 than syngeneic cardiac grafts. More importantly,expression of CXCR6 in CD8+ T cells was also up-regulated by allograft rejection. However, injection of anti-CXCL16 mAb could not inhibit cytotoxic activity of CD8+ T cells. Moreover, experimental group could not prolong the cardiac graft survival time as compared with control group. Conclusion Expression of CXCR6 in CD8+ T cells is up-regulated in allograft rejection.
