Effects of transfected gut-enriched Krüppel-like factor on growth of human gastric carcinoma cell line SGC-7901 and its xenograft in nude mice
10.3760/cma.j.issn.0254-1432.2011.01.008
- VernacularTitle:胃肠富集Krüppel样因子基因转染对人胃癌细胞株SGC-7901及裸鼠移植瘤生长的影响
- Author:
Hui YAN
;
Weichang CHEN
;
Jiannong CEN
;
Hongjie SHEN
;
Xiaofei QI
- Publication Type:Journal Article
- Keywords:
Gut-enriched Krüppel-like transcription factors;
Transfection;
Stomach neoplasms;
Cell line,tumor;
Mice,nude;
Neoplasm transplantation
- From:
Chinese Journal of Digestion
2011;31(1):30-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumour effects of transfected gut-enriched Krüppellike factor(GKLF) on human gastric carcinoma cell line SGC-7901 in vitro and in vivo. Methods The expression of GKLF mRNA and protein in human gastric carcinoma cell line SGC-7901 were detected before and after transfection by real-time fluorescence quantitative PCR and Western blot,respectively. Proliferation and invasion in SGC-7901 were measured respectively by MTT assay, flow cytometry, colony formation assay and cell invasion assay after transfected with GKLF. The growth of xenograft was observed, the microvessel density(MVD) of xenograft tissue was determined by immunohistochemistry. Results The GKLF mRNA and protein in SGC-7901 were overexpressed after transfected with GKLF(P＜0.05). The proliferative speed of SGC7901-pcDNA3.1-GKLF group was markedly lower than that of SGC-7901 and SGC7901-pcDNA3.1 groups (P＜0.05). Transfected with GKLF caused part of the G0/G1 arrest, decreased clone formation rate and the invasion ability (P＜0.05). The growth speed of xenograft in SGC7901-pcDNA3.1-GKLF group was lower, the weight and MVD of xenograft tissue in SGC7901-pcDNA3. 1-GKLF group were less (P＜ 0. 05).Conclusion Transfected with GKLF maysuppress proliferation and invasion in human gastric carcinoma cell line SGC-7901, inhibit the growth and the angiogenesis of xenograft in nude mice.
