The expression of protease-activated receptors in colon mucosa tissue of patients with diarrhea-predominant irritable bowel syndrome and its significance

VernacularTitle:蛋白酶激活受体在腹泻型肠易激综合征患者结肠黏膜组织中的表达及意义
Author: Ruihui GENG ; Jun GAO ; Wenbin WU ; Lei WEI ; Lining XIAO ; Shude LI ; Duowu ZOU ; Zhaoshen LI
Publication Type:Journal Article
Keywords: Receptors,proteinase-activated; Irritable bowel syndrome; Intestinal mucosa; Gene expression; Diarrhea
From: Chinese Journal of Digestion 2011;31(4):239-242
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of protease-activated receptors (PARs) in colon mucosal tissue of patients with diarrhea-predominant irritable bowel syndrome (D-IBS). Methods Colon mucosa tissues were obtained from 28 D-IBS patients and 18 normal controls by colonoscopy.The expression of PARs was detected using Western blotting and real-time PCR. Data were analysed by SPSS 18. 0 softwave, and comparisons between groups were done using Mann-Whitney U test.Results There was no difference in expression of PAR1 between D-IBS patients and normal controls (P=0. 300). The expressions of PAR2 and PAR4 were higher in D-IBS patients than those in normal controls (0.99±0.67 vs 0.63±0.38, P=0.038 and 0.37±0. 14 vs 0.25±0. 11, P=0.013,respectively). The results of real-time PCR were in accordance with those of Western blotting.Conclusion The increased expressions of PAR2 and PAR4 in colon mucosa of D-IBS patients indicate that these two PAR receptors may be involved in the process of D-IBS.