Study on the time of amounted to peak of human adipose-derived stromal cells differentiation into neural precursor cells in vitro
10.3760/cma.j.issn.1674-6554.2011.04.005
- VernacularTitle:成人脂肪基质细胞体外诱导分化为神经前体细胞的数量达峰时间研究
- Author:
Yanan CAI
;
Xiaodong YUAN
;
Ya OU
;
Changqing YE
;
Hui LIU
- Publication Type:Journal Article
- Keywords:
Adult adipose-derived stromal cell;
β-Mercaptoethanol;
Induced differentiation;
Neural precursor cells;
Ultrastructure
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2011;20(4):302-305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reseach the time point of the highest percentage of neural precursor cells derived from adipose stromal cells (ADSCs) in vitro, and to observe the ultrastructure features of neural precursor cells. Methods Used the β-mercaptoethanol to induce ADSCs to differentiate into neural precursor cells and neuron-like cells. The morphology of the uninductedcells and inducted cells were observed with inverted phase contrast microscope. The expression of nestin which was the marker of neural precursor cell in each group was detected using immunofluorescence staining method. The ultrastructural feature of cells which was induced for 3 hours were observed. Results The highest ratio of positive expression of nestin was 3 hours following induction,with the ratio ( 86.25 ± 4.82) %. There were many protuberance on the cell membrane under transmission electron microscopy.There were plenty of organelles in the neural precursor cells. The neural precursor cells had a large size nucleus,large nucleoplasmic index, much extended chromatin,and less condensed chromatin. The nucleus had double-layer nuclear envelope, more nuclear pore on the nuclear envelope. Conclusion The time point of the highest percentage of neural precursor cells derived from ADSCs is 3 hours,and the ultrastructral feature of induced neural precursor cells confirm that cells at this time point are in a state of split active period.
