Effects of hyperbaric oxygen on the expression of hypoxia inducible factor-1α mRNA and vascular endothelial growth factor mRNA in colon adenocarcinoma cells
10.3760/cma.j.issn.0254-1424.2011.02.003
- VernacularTitle:高压氧对结肠癌细胞缺氧诱导因子-1α和血管内皮生长因子mRNA表达的影响
- Author:
Lida ZHANG
;
Li MA
;
Xiaoqiang TIAN
;
Wei ZHANG
;
Ping HUANG
;
Xia QIAN
;
Lin YANG
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Adenocarcinoma;
Hypoxia inducible factor-1 α;
Vascular endothelial growth factor
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2011;33(2):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hyperbaric oxygen (HBO) on the expression of hypoxia inducible factor-1α (HIF-1α) mRNA and vascular endothelial growth factor (VEGF) mRNA in colon adenocarcinoma cells. Methods SW480 colon cancer cells were divided into a control group and an HBO group, which was in turn divided into various subgroups according to the various HBO pressures (0.15 MPa, O. 20 MPa, 0.25 MPa) and exposure times (12, 24 and 36 h) tested. The expression of HIF-1α and VEGF mRNA was evaluated with RT-PCR after the various treatments. Results The expression of HIF-1α mRNA was lower in the SW480 cells than in the controls after exposure to 0.25 MPa HBO for 12 h. No expression of HIF-1α mRNA was detected after exposure to 0.25 MPa HBO for 24 h. The expression of VEGF mRNA was lower after exposure to 0.25 MPa HBO for 36 h than in the controls. Conclusion Absence of HIF-1α mRNA expression and decreased expression of VEGF mRNA in SW480 can be observed after exposure to 0.25 MPa HBO for 24 h, suggesting that HBO may inhibit the formation of tumor blood vessels through down-regulating the expression of these mRNAs.
