Expression of SCCA1 and SCCA2 in cervical squamous cell carcinoma
10.3760/cma.j.issn.1006-9801.2011.03.004
- VernacularTitle:子宫颈鳞状细胞癌中SCCA1和SCCA2的分子表达及其意义
- Author:
Lijuan YAN
;
Xin ZHAO
;
Shuli SHAO
;
Sutang GUO
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Neoplasms,squamous cell;
SCCA1;
SCCA2;
Real-time RT-PCR
- From:
Cancer Research and Clinic
2011;23(3):157-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression levels of SCCA1 and SCCA2 mRNA in tissues of cervical squamous cell carcinoma. To investigate the role of this gene in the clinical diagnosis, evaluation of treatment and observation of prognosis of cervical squamous cell carcinoma. Methods Quantitative real-time RT-PCR was used to detect the expression of SCCA1 and SCCA2 mRNA in tissues of 60 cases of cervical squamous cell carcinoma and those of 30 cases of normal cervical tissues. Results The expression level of SCCA2 mRNA in tissues of 30 cases of cervical squamous cell carcinoma was higher than in those of 15 cases of normal cervical tissues (4.405 ± 2.310, 9.088 ± 2.195) (t =-6.513, P ＜0.001), while the expression level of SCCA 1 mRNA did not significantly differ between normal and malignant tissues (P ＞0.05). The expression of SCCA2 mRNA was relevant to FIGO stages and there was a tendency for this gene to increase with the stage getting worse (F =8.313, P ＜0.05). Moreover, the overexpression of SCCA2 mRNA was significantly correlated with lymph node metastases (t =2.853, P ＜0.05). The expression of SCCA2 mRNA was not correlated with age and pathological grading (P ＞0.05). However, the expression of SCCA1 mRNA was not correlated with age,FIGO stages, lymph node metastases and histological grade (P ＞0.05). Conclusion The expression of SCCA2 mRNA may provide help for more accurate diagnosis on the clinical stages and lymph node metastases of cervical squamous cell carcinoma.
