ODNSIRUCTION AND ANALYSIS OF cDNA LIBRARY OF NECATOR AMERICANUS THIRD STAGE LARVAE
- VernacularTitle:美洲钩虫第三期幼虫Cdna库的构建及分析
- Author:
Bin ZHAN
;
Hawdon JOHN
;
Qiang SHAN
;
Hainan REN
;
Huiqing QIANG
;
Shuhua XIAO
- Publication Type:Journal Article
- Keywords:
Necator americanus;
cDNA library;
ESTs
- From:
Chinese Journal of Parasitology and Parasitic Diseases
2000;18(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To obtain the genetic information on Necator americanus and to search for the purpose genes.[Methods] Mrna was isolated from the third stage larvae of Necator americanus maintained in hamsters.Double strand Cdna was synthesized and ligated to ΛzapⅡ vector to construct the Cdna library.Expresed se-quence tages (ESTs) were obtained by single pass sequencing of randomly isolated Cdna clones from the es-tablished library.[Results] A Cdna librazy of N.americanus was successfully constructed with high recombi-nant efficiency.The titer of unamplified library was 1×107.The insert size was about 750～3000bp.Of 11 ESTs obtained from the library,7 have a significant homology with certain functional genes.[Conclunsion]A high quality and high representative Cdna library of N.americanus was constructed at the first time and ome functional genes were identified from the library by ESTs.
