Protective Effects of Epigallocatechin Gallate After UV Irradiation of Cultured Human Lens Epithelial Cells.
10.3341/kjo.2008.22.3.183
- Author:
Jun HEO
1
;
Byung Rae LEE
;
Jae Woong KOH
Author Information
1. Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea. clearcornea@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Cell viability;
Cultured human lens epithelial cells;
Epigallocatechin gallate (EGCG);
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay;
UV irradiation
- MeSH:
Antioxidants/*pharmacology;
Catechin/*analogs & derivatives/pharmacology;
Cell Count;
Cell Survival/drug effects;
Cells, Cultured;
Coloring Agents/diagnostic use;
Dose-Response Relationship, Drug;
Epithelial Cells/radiation effects;
Humans;
Lens, Crystalline/cytology/*radiation effects;
Radiation Injuries/*prevention & control;
Radiation-Protective Agents/*pharmacology;
Tetrazolium Salts/diagnostic use;
Thiazoles/diagnostic use;
*Ultraviolet Rays
- From:Korean Journal of Ophthalmology
2008;22(3):183-186
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To evaluate the protective effects of epigallocatechin gallate (EGCG) against UV irradiation of cultured human lens epithelial cells. METHODS: We irradiated cultured human lens epithelial cells with a 30-second pulse from a UV lamp with an irradiance of 0.6 mW/cm2. Five minutes and 1 hour after UV irradiation, we administered 0, 5, 10, 15, 25, 50, or 100 uM EGCG. The cell number was measured with a microscopic counting chamber and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: Compared to untreated cells, the total number of cultured human lens epithelial cells was markedly higher after UV irradiation. In a dose-dependent manner, viability was also higher in EGCG-treated cells. CONCLUSIONS: EGCG increased the cell count and cell viability after UV irradiation of cultured human lens epithelial cells, indicating that EGCG can protect lens epithelium against UV damage.
