Lipopolysaccharide Down-regulates SR-BI Expression in a Nucleus Factor-κB Pathway-dependent Manner
10.3760/cma.j.issn.1008-6706.2011.08.002
- VernacularTitle:脂多糖对B类Ⅰ型清道夫受体表达的调节
- Author:
Dongli CAO
;
Lijin WANG
;
Tao JIN
;
Hui CHEN
- Publication Type:Journal Article
- Keywords:
Scavenger receptor class B type Ⅰ;
Lipopolysaccharide;
Nucleus factor-κB
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(8):1011-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of cholesterol efflux,the scavenger receptor class B type Ⅰ(SR-BI) protein expression in THP-1 maerophage derived foam cells treated with Lippolysaecharide (LPS), and to discover the role of NF-κB pathway in this process.Methods The foam cells were treated with LPS along or treated with N-p-Tosyl-L-phenylalanine chloromethyl ketone(TPCK) for 24 h.The protein levels of SR-BI and intranuclear NF-κB p65 were measured by Western blotting.Cellular lipid accumulation was determined by high performance liquid ehromatograpby analysis.Cholesterol efflux was determined by FJ-2107P type liquid scintillator.Results The expression of SR-BI was decreased after treated with LPS,while the intranuclear NF-κB p65 protein level was increased by LPS.The results also showed that cellular lipid accumulation was increased ,while the cellular cholesterol efflux was decreased in THP-1 maerophage derived foam cells after exposed to LPS for 24 h and these changes can be reversed partly by pretreatment with TPCK.Conclusion LPS could down-regulate the expression of SR-BI, promote the accumulation of lipid and decrease cellular cholesterol efflux in THP-1 maerophage derived foam cells ,which should be related to the TLR4/NF-κB dependent pathway.
