The effects of intensive insulin treatment on islet β cell apoptosis associated protein bcl-2 and bax in type 2 diabetic rats
10.3760/cma.j.issn.1008-6706.2011.08.001
- VernacularTitle:强化胰岛素治疗对糖尿病大鼠胰岛β细胞凋亡蛋白bcl-2和bax的影响
- Author:
Defeng WANG
;
Li SUN
;
Huifen ZUO
;
Chun DENG
;
Hong LIU
;
Haitao WANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus type 2;
Rats;
Intensive insulin treatment;
Islet of Langerhans;
Apoptosis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(8):1009-1011
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of intensive insulin treatment on islet β cell apoptosis associated protein bcl-2 and bax in type 2 diabetic rats.Methods 36 Wistar rats were randomly divided into two groups : normal control group and high fat diet group.Rats in normal control group fed by basical feedstuff.Rats in high fat diet group fed by high fat and basical feedstuff.After 10 days,rats in high fat group were injected with STZ.After 3 days,rats in high fat group were randomly divided into two groups:diabetes control group and insulin treatment group.The course of treatment was 4 weeks.After 10 days by fat milk intragastfic administration, after 3 days of STZ injection and after 4 weeks treatment, each index was measured.After experiment, pancreatic tissue bel-2 and bax were detected through immunohistocbemical method.Results After 4 weeks intensive insulin treatment,the bcl-2 was significantly increased at(6.20 ± 2.05 )% in insulin treatment group than diabetes control group.The bax was significantly decreased at ( 2.68 ± 1.04 ) % in insulin treatment group than diabetes control group ( P < 0.05 ).Conclusion The method of insulin intensive treatment could increase islet βcell bcl-2 and decrease bax in type2 diabetic rots, Insulin intensive treatment could decrease islet β cell apoptosis.
