Radiation-Induced changes in expression of genes related to DNA damage response in lymphocytes of human peripheral blood
10.3760/cma.j.issn.0254-5098.2011.02.004
- VernacularTitle:辐射诱导人外周血淋巴细胞DNA损伤反应相关基因表达变化的实验研究
- Author:
Haizhuo GUO
;
Zhongzhi QI
;
Ning WU
;
Juancong DONG
;
Shunzi JIN
- Publication Type:Journal Article
- Keywords:
Radiation;
Human peripheral blood lymphocytes;
Cdknla;
Gadd45α;
Real-time qPCR;
Biomarker
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of DNA damage response genes induced by radiation in human peripheral blood lymphocyte,and to explore the new biomarkers of radiation.Methods The human peripheral blood cells were irradiated to X-rays at different doses of 0,1,2,3,4,and 5 Gy.The quantitative real.time qPCR wag used to detect the expressions of cyclin-dependent kinase inhibitor l a gene(Cdknl a)and growth arrest and DNA damage inducible gene(Gadd45a)in lymphoeytes at 4 and 24 h post-irradiation,respectively.The method of CB mieronucleus was used to determine the change of micronucleus ratio.Results The expression of Cdknl a in peripheral blood lymphocytes wag increased significantly at 4 and 24 h post-irradiation to 0-5 Gy.reached the peak at 4 Gy and began to decrease at 5 Gy,which showed a dose-dependent manner(r=0.946,0.975,P<0.05).Similarly,the expression of Gadd45α in human peripheral blood lymphocytes was also increased significantly at 4 and 24 h post-irradiation to 0-5 Gy in a dose-dependent manner,while the expression of Gadd45a at 4 h wag higher than that at 24 h(r=0.936,0.797,P<0.05).The ratio of micronuclei wag increased significantly at 4 and 24 h post-irradiation to 0-5 Gy(r=0.990,0.984,P<0.05).Conciusions Cdknl a and Gadd45α expression could be increaged significandy at 4 and 24 h post-irradiation to 0-5 Gy,showing a good linear relationship.which might be candidate for radiation biological dosimeter.
