Human lymphocyte damage and phosphorylation of H2AX and ATM induced by γ-rays
10.3760/cma.j.issn.0254-5098.2011.02.003
- VernacularTitle:γ射线诱导人淋巴细胞损伤及磷酸化组蛋白H2AX和ATM表达
- Author:
Mei TIAN
;
Yan PAN
;
Jianxiang LIU
;
Jianlei RUAN
;
Xu SU
- Publication Type:Journal Article
- Keywords:
60Co;
γ-rays;
Lymphocyte;
γ-H2AX;
ATM phosphorylation;
Micmnucleus
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(2):126-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate 60Co γ-ray induced damage in lymphocytes and the relationship between doses of 60Co γ-ray irradiation and the levels of phosphorylated H2AX and ATM.Methods Cells were irradiated with 60Co γ-rays in the range of 0-8 Gy.The levels of phosphorylated H2AX and ATM were detected by Western blot and FACScan,respectively.The micronucleus(MN)was analyzed by CB method to evaluate DNA damage.Results FACScan results showed the dose-effect relationship of γ-H2AX expression were linear.square at 0.5 h post-irradiation to different doses,and the fitting curve was shown as Y=3.96+11.29D-0.45D2.The level of phosphorylated ATM(p-ATM)was not changed significantly by using the same method.Western blot showed that p-ATM protein expression was significandy increased after irradiation compared with sham.irradiated group.The MN assay which represented DNA damage was sensitive to different doses.Conclusions γ-ray irradiation could induce the phosphorylation of H2AX and ATM,which may play an important role in indicating DNA damage.Both of H2AX and ATM have the potential as sensitive biomarker and biodosimeter for radiation damage.
