Correlation analysis on MN-RET from peripheral blood and MN-PCE from bone marrow in mice following exposure to irradiation
10.3760/cma.j.issn.0254-5098.2011.02.002
- VernacularTitle:辐射诱导小鼠外周血网织红细胞微核与骨髓嗜多染红细胞微核的剂量-效应关系
- Author:
Libo LIU
;
Jian ZHANG
;
Yuguang LIU
;
Guanying NI
;
Shuzheng LIU
- Publication Type:Journal Article
- Keywords:
Radiation;
Mice;
Retieulocyte micronucleus;
Polychromatic erythrocyte micronucleus;
Flow cytometry;
Biodosimeter
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(2):122-125
- CountryChina
- Language:Chinese
-
Abstract:
objective To study the changes of reticulocyte micronueleus(MN-RET)from peripherai blood and polychromatic erythrocyte mieronucleUS(MN-PCE)from bone marrow in mice following exposure to X-rays in order to provide an experimental basis for exploring possible hish-throughput radiation biodosimeter.Methods Male ICR mice were whole-body irradiated with 0,0.5,1,2,4 and 5 Gy at a dose rate of 0.488 Gy/min.MN-RET from peripheral blood wag scored with FCM and MN-PCE from bone marrow was scored with manual microscopy at 24,48 and 72 h post-irradiation.Results Both MN-RET and MN-PCE rates increaged with doses in the range of 0-5 Gy at 24,48 and 72 h after WBI.The dose-response relationship can be fit with linear equations(t=10.26-25.77,P<0.05).The correlation coeffcients between MN-RET from peripheral blood and MN-PCE from bone mallow were highly significant(r=0.986-0.996,P<0.05).Conclusions In view of its simplicity,accuracy and high throughput capacity,FCM scoring of peripheral blood MN-RET may be a candidate for radiation biodosimetry,More work should be carried out on human specimens to investigate this possibility.
