Effects of X-ray irradiation on expression of Pokemon gene in A549 cells of human lung adenocarcinoma

VernacularTitle:X射线对人肺腺癌A549细胞Pokemon基因表达的影响
Author: Lu WANG ; Yue ZOU ; Qisheng JIANG ; Wei LI ; Xiujun SONG ; Xiangyan ZHOU ; Cuilan WANG
Publication Type:Journal Article
Keywords: Lung cancer; Pokemon gene; A549 cells; X-rays; Radiation
From: Chinese Journal of Radiological Medicine and Protection 2011;31(2):177-179
CountryChina
Language:Chinese
Abstract: Objective To study the dose-and time-effects of X-ray irradiation on the expression of Pokemon gene in A549 cells of human lung adenocarcinoma.Methods A549 cells were cultured in vitro and exposed to X-rays with the doses of 2,4,6 and 8 Gy,respectively.Untreated A549 cells were used as control group.The relative levels of Pokemon mRNA expression in the cells were detected by using quantitative real-time PCR at 2,4,8,12,24,48 and 72 h after irradiation.Results The Pokemon mRNA expression levels decreased in the early period after irradiation(except 2 and 4 h after irradiation in 2 Gy group)and then increased in the later stage(48 h after irradiation)with significant statistical differences at the most time points in comparison with the control group(t=3.40-154.76,P<0.05).Conclusions Higher doses of X-rays may degrade the expression of Pokemon mRNA in the human A549 cells and induce apoptosis in the early period,hut also may upgrade its expression in the later period, which might be correlated with the cell cycle regulation and DNA damage repair in the A549 cells.