Effect of sevoflurane on Survivin expression in human adenocarcinoma cell line A549
10.3760/cma.j.issn.0254-1416.2011.02.012
- VernacularTitle:不同浓度七氟醚对人肺腺癌A549细胞Survivin蛋白表达的影响
- Author:
Hua LIANG
;
Chengxiang YANG
;
Xianjie WEN
;
Qiaoling ZHOU
;
Miaoning GU
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Cell line,tumor;
Inhibitor of apoptosis proteins;
Sevoflurane
- From:
Chinese Journal of Anesthesiology
2011;31(2):173-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of sevoflurane on Survivin expression in human adenocarcinoma cell line A549. Methods A549 cells were obtained from Shanghai Cell Biology Medical Research Institute, Chinese Academy of Sciences and inoculated in 96 well culture plate. After being cultured for 24 h, the cells were randomly divided into 4 groups: Ⅰ , Ⅱ , Ⅲ and Ⅳ groups exposed to 95 % O2 -5 %CO2,1.7%, 3.4% and 5.1% sevoflurane respectively. A549 cells were exposed to sevoflurane for 2, 4 and 6 h respectively and then cultured for another 48 h in Ⅱ , Ⅲ and Ⅳ groups. Proliferation of A549 cells were measured by methyl thiazolyl tetrazolium (MTT) assay, and apoptosis was detected with flow cytometer at 48 h after 2, 4 and 6 h sevoflurane exposure. The expression of Survivin in A549 cells was determined by Western blot analysis at 48h after 4 h sevoflurane exposure. Results The rate of proliferation inhibition and percentage of apoptotic cells were significantly higher while the expression of Survivin was significantly lower in a concentration-dependent manner in Ⅱ , Ⅲ and Ⅳ groups as compared with group Ⅰ . Conclusion Sevoflurane can inhibit proliferation and induce apoptosis of A549 cells by inhibition of Survivin expression.
