Effects of silencing of iASPP gene on human bladder cancer cells

VernacularTitle:RNA干扰下调p53抑制因子iASPP表达对膀胱癌细胞的影响
Author: Tao LIU ; Lin LI ; Hui JIA ; Hongwei JING ; Chuize KONG
Publication Type:Journal Article
Keywords: RNA interference; iASPP; Tumor suppressor protein p53; Urinary bladder neoplasms; Carcinoma
From: Chinese Journal of Urology 2011;32(4):239-243
CountryChina
Language:Chinese
Abstract: Objective To discuss the effects of silencing of iASPP gene on human bladder cancer cells. Methods RNAi silencing of iASPP gene in bladder cancer cell 5637 and T24 cells were used by lentiviral mediated interfering short hairpin RNAs. Cell proliferation was tested by MTT assay, and rate of colony was tested by colony formation assay. Cell cycles were tested by using fluorescence-activated cell sorting. Results Down-regulation of iASPP could inhibit the growth and proliferation of human bladder cancer cells (P＜0.05). iASPP know-down could decrease the colony formation of 5637 and T24 cells (P＜0, 05). Knocking down of iASPP in 5637 and T24 cells showed cell arrested at G1. Conclusions Silencing of iASPP gene could inhibit proliferation and colony formation of bladder cancer, iASPP might be an important target for gene therapy of bladder cancer.