A comparison study on the detection of anti-human immunodeficiency virus type 1 (HTV-1) antibodies in different populations with a new rapid test using oral mucosal transudate samples versus enzyme linked immunosorbent assay (ELISA) using serum samples
10.3760/cma.j.issn.0412-4030.2011.05.003
- VernacularTitle:口腔黏膜渗出液快速诊断试剂与血清ELISA试剂检测不同人群HIV-1抗体的评价
- Author:
Yan WU
;
Kerong WANG
;
Jing HAN
;
Hongxin ZHAO
;
Hui ZENG
;
Keyi XU
;
Yanchun LIU
;
Huiwen YAN
;
Xingwang LI
;
Wenhui LUN
- Publication Type:Journal Article
- Keywords:
HIV antibodies;
Mouth mucosa;
Exudates and transudates;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Dermatology
2011;44(5):302-305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the consistence in the detection of antibodies against HIV-1 between a new rapid test using oral mucosal transudate (OMT) samples and ELISA using serum samples. Methods Two-hundred patients who were positive for anti-HIV-1 antibodies by serum ELISA and confirmed by Western blot to be infected with HIV, and 600 healthy human controls negative for anti-HIV-1 antibodies by serum ELISA, were eligible for this study. OMT samples were collected from these subjects and subjected to a rapid test for anti-HIV-1 antibodies. The factors influencing the performance of the rapid test were analyzed. Results Of the 200 OMT specimens from HIV-infected patients, 198 showed positive reaction, 2 showed negative reaction. Among the 198 positive reactions, 192 (96%) were "clear" and easy to make decisions, 4 (2%) were "faint", 2(1%) were "very faint" and required professionals to make decisions. The rapid test was negative in all the 600 OMT specimens from the control group. Conclusions The consistence in the detection of anti-HIV-1 antibodies between the OMT rapid test and serum ELISA was 99% in HIV-positive specimens, 100% in HIV-negative specimens, and 99.75% in all the specimens.
