Research on the effect of statins on insulin secretion from pancreatic islet in rats and its mechanisms
10.3760/cma.j.issn.0578-1426.2011.05.009
- VernacularTitle:他汀类调脂药对大鼠胰岛胰岛素分泌的影响及机制研究
- Author:
Baocheng CHANG
;
Miaoyan ZHENG
;
Chunyan SHAN
;
Juhong YANG
;
Ying WANG
;
Huizhu REN
;
Liming CHEN
;
Peihua FANG
- Publication Type:Journal Article
- Keywords:
Islets of Langerhans;
Insulin secretion;
Atorvastatin;
Fluvastatin
- From:
Chinese Journal of Internal Medicine
2011;50(5):393-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the inhibitory effect of statins on glucose-stimulated insulin secretion (GSIS) of pancreatic islet in rat and to explore its mechanisms. Methods According to the average volume, freshly isolated or 24-hour cultured pancreatic islets were randomly divided into control group( incubated with Kreb-Ringer bicarbonate buffer), the atorvastatin group( incubated with 100 μ mol/L atorvastatin), the fluvastatin group (incubated with 100 μ mol/L fluvastatin)and the pravastatin group (incubated with 100 μ mol/L pravastatin). Stimulated by 2. 8,5. 5,11.1,16. 7 mmol/L and 25.0 mmol/L glucose respectively, the effect of 100 μ mol/L statins on ATP content and GSIS was compared in the four groups. GSIS was performed by the 37℃ bath incubation method and ATP content was measured by chemiluminescence method. Results Incubated with 100 μ mol/L atorvastatin for 30 minutes, in the presence of 16. 7 mmol/L glucose, the ATP content [(9. 54 ± 1. 64) pmol/islet vs ( 12. 33 ± 1.89) pmol/islet] and GSIS (1.60 ± 0. 21 vs 2. 39 ± 0. 30) were significantly reduced in comparison with the control group (P＜0. 05). Cultured with 100 μmol/L fluvastatin for 24 hours, the ATP content [( 10. 24 ±2.01 )pmol/islet vs (12. 31 ±2. 16) pmol/islet] and GSIS (3. 12 ± 0. 32 vs 4. 17 ±0. 37 ) were all significantly decreased at the higher glucose concentration of 16. 7 mmol/L ( P ＜ 0. 05). Conclusion Atorvastatin and fluvastatin may inhibit GSIS by decreasing ATP content in pancreatic islet and the inhibitory effect is related to the strength of its lipophilicity.
