Effect of silencing STAT3 gene on growth of human pancreatic cancer cells in vivo
10.3760/cma.j.issn.1007-631X.2011.04.019
- VernacularTitle:沉默STAT3基因对人胰腺癌细胞体内生长能力的影响
- Author:
Chen HUANG
;
Zhengjun QIU
;
Tao JIANG
;
Lin ZHU
;
Jun CAO
;
Kejian HUANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
RNA interference;
STAT3 transcription factor
- From:
Chinese Journal of General Surgery
2011;26(4):324-327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of RNAi-mediated STAT3 gene silence on human pancreatic cancer cells growth in vivo. Methods STAT3 shRNA expression vector was stably transfected to SW1990 cells. STAT3 and p-STAT3 protein was examined using Western blot. The growth ability of SW1990 cells in vivo was determined in a subcutaneous tumor model of nude mice. Western blot was performed to detect the protein expression of Bcl-xL and cyclin D1. Results The protein expression of STAT3 and p-STAT3 decreased by 90% and 92% by stable transfection of STAT3 shRNA expressing vectors(P ＜0. 05). Inhibition of STAT3 with RNAi significantly inhibited the growth ability of SW1990 cells in vivo( P ＜ 0. 05 ). The tumor weight significantly decreased( P ＜ 0. 05 ). Moreover, the relative Bcl-xL and cyclinD1 protein expression in SW1990-RNAi cells reduced by 56% and 50% compared with that of the parental SW1990 cells, respectively (P ＜ 0. 05). Conclusions Inhibition of STAT3 with RNAi significantly inhibits the growth ability of pancreatic cancer cells through down-regulating Bcl-xL and cyclin D1.
