Effect of natural killer cells treated by serum of severe preeclampsia patient on apoptosis and endothelin-1 secretion of endothelial cells
10.3760/cma.j.issn.1007-9408.2011.05.007
- VernacularTitle:重度子痫前期患者血清作用下自然杀伤细胞对血管内皮细胞功能的影响
- Author:
Jun WEI
;
Lin LIN
;
Yu SUN
;
Zhiying YANG
;
Tao SHANG
- Publication Type:Journal Article
- Keywords:
Pre-eclampsia;
Killer cells,natural;
Endothelium,vascular;
Apoptosis;
Endothelin-1
- From:
Chinese Journal of Perinatal Medicine
2011;14(5):289-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of natural killer (NK) cells treated by serum of severe preeclampsia patient on the function of endothelial cells.Methods Fifteen patients with severe preeclampsia and 15 normal pregnant women from the Obstetrics department,Shengjing Hospital,China Medical University were admitted into this case-control study from January 1,2006 to December 31,2008.NK cells from healthy non-pregnant woman were incubated with 20% serum from severe preeclampsia patients or normal pregnant women for 20 hours.Then,human umbilical vein endothelial cells ( HUVEC) and serum-treated NK cells were co-incubated for 24 hours.Apoptosis of HUVEC was checked by flow cytometry and electronic microscope.Endothelin-1 (ET-1) levels in the supernatants of HUVEC and NK cells were examined by radioimmunologic method.Results In severe preeclampsia group,the percentage of early apoptosis cell (Annexin V-FITC+ /PI+ ) was (23.81±4.79)%,that of late apoptosis cell (AnnexinV-FITC+/PI+ ) was (3.29±1.04) %,while those were (16.59±5.13)% and (2.24±0.72)% respectively in normal pregnant group (P＜0.01).There was no significant difference in dead cells (Annexin V-FITC- /PI+ ).Under electronic microscope,typical morphologic changes of apoptosis were shown in severe preeclampsia group.Level of ET-1 in
