β-lactamase genotyping of multi-drug resistant Acinetobacter baumannii in a neonatal intensive care unit
10.3760/cma.j.issn.1007-9408.2011.05.001
- VernacularTitle:新生儿重症监护病房多重耐药鲍曼不动杆菌β-内酰胺酶的基因型
- Author:
Gangfeng YAN
;
Yun CAO
;
Di QU
;
Chuanqing WANG
;
Chao CHEN
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Drug resistance,bacterial;
beta-lactamases;
Genotype;
Intensive care units,neonatal
- From:
Chinese Journal of Perinatal Medicine
2011;14(5):257-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between drug resistance and β-actamase genes of multi-drug resistance Acinetobacter baumannii (MDR-AB) in neonatal intensive care unit to provide evidence for rational antibiotics administration and nosocomial infection control.Methods Twenty-six MDR-AB strains were separated and collected from clinical specimens.The minimum inhibitory concentrations of 13 antimicrobial agents were determined by agar dilution method.Genotypes of β-lactamase were detected by polymerase chain reaction.Results The resistant rates of the 26 strains to Ceftazidime,Cefoxitin,Piperacillin-tazobactam and Ciprofloxacin were 100.0%.About 80.8% to 96.2% of these strains were resistant to the other antimicrobial drugs.Among the 26 MDR-AB strains,100% (26/26) strains possessed oxa-51,77% (20/26) possessed oxa-23 gene,54% (14/26) carried arnpC gene,both oxa-23 and ampC were identified in 42% (11/26) strains,while oxa-24,oxa-58,imp-1,imp-4 and vim-2 gene were not identified.Conclusions The drug resistance of Acinetobacter baumannii is serious,oxa-23 and ampC are the major plactamase genes carried by MDR-AB in neonatal intensive care unit.
