Intervention of azithromycin on adriamycin-induced nephropathy rats
10.3760/cma.j.issn.1001-7097.2011.03.016
- VernacularTitle:阿奇霉素对大鼠阿霉素肾病的干预作用
- Author:
Bili ZHANG
;
Lanyun SONG
;
Wenhong WANG
;
Xuan ZHANG
;
Shuying FAN
;
Li YANG
- Publication Type:Journal Article
- Keywords:
Azithromycin;
Nephrotic syndrome;
Intercellular adhesion molecule 1;
Prednisone;
Nephrin;
Podocalyxin
- From:
Chinese Journal of Nephrology
2011;27(3):203-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of azithromycin on serum indicators, urine indicators, renal pathology, intercellular adhesion molecule-1 (ICAM-1), nephrin and podocalyxin in adriamycin(ADR)-induced nephropathy(ADN) rats. Methods ADR nephropathy was induced by a tail intravenous injection of ADR. The rats were randomly divided into azithromycin-treated group, prednisone -treated group, integrated treatment group, model group and control group. Serum index and 24 h urine protein were measured serially at 0, 4th, 8th weeks. The values of creatinine clearance (Ccr) were calculated. Kidney tissues were collected for microscopy observation. The expressions of nephrin, podocalyxin and ICAM-1 in renal tissue were detected by immunohistochemistry SP. Results Compared with normal control group, at the 4th week, 24 hours urine protein and albumin reached the level of ADR nephropathy model. Compared with model group, at the 8th week, 24 h urine protein, cholesterol, serum creatinine, renal pathology changes in the three treated group rats were significantly reduced (P<0.05), serum total protein, albumin and Ccr (except C group) significantly raised (P<0.05), ECM/GA and renal pathology score significantly reduced (P<0.01), ICAM-1 significantly decreased (P<0.01), nephrin and podocalyxin significantly increased. Besides, the curative effect of integrated treatment was better than other treatment group. Conclusions Azithromycin induces similar responses in ADR nephropathy as prednisone, but its early protective effect of renal function is worse than prednisone.The integrated treatment of azithromycin and prednisone has a synergistic effect, and the efficacy is superior to each drug alone.
