Influence of viper venom nerve growth factor on expressing of candidate plasticity-related gene 15 and nuclear factor of kappa B in rat brain tissue after cerebral ischemia reperfusion injury
10.3760/cma.j.issn.1006-7876.2011.06.005
- VernacularTitle:蛇毒型神经生长因子通过影响候选可塑性相关基因15和核转录因子κB的表达促进脑缺血再灌注损伤后神经重塑
- Author:
Shengliang SHI
;
Sen LIANG
;
Shijian CHEN
;
Xin LI
;
Guinan BI
;
Tangwei LIU
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Reperfusion injury;
Nerve growth factor;
Membrane proteins;
Nerve tissue proteins;
NF-kappa B;
Polymerase chain reaction
- From:
Chinese Journal of Neurology
2011;44(6):389-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance and mechanism of intracerebroventricular injection viper venom nerve growth factor (Vngf) in rat neural plasticity after cerebral ischemia reperfusion injury.Methods Ninety Wistar male rats were randomly assigned into Vngf-25 U group (n = 18), Vngf-50 U group (n = 18), Vngf-100 U group (n = 18), ischemia reperfusion group (n = 18) and sham operated group.The expression of candidate plasticity-related gene 15(cpg-15) Mrna and nuclear factor of kappa B ( NF-Κb ) Mrna in rat brain tissues which were collection at 2,7,14 days after surgery were evaluated by the real time PCR.Results The expression of cpg-15 Mrna and NF-Κb Mrna began to increase after surgery( the F value of cpg-15:70.43, 34.11, 31.89, the F value of NF-Κb: 27.47, 34.56, 31.89,P＜0.01).At the same time, expression of cpg-15 Mrna and NF-Κb Mrna in the Vngf groups was significantly different from the I/R group and the sham operated group (the F value of cpg-15:48.18, 55.93, 78.43, the F value of NF-Κb: 45.92, 55.72, 50.49, P ＜0.01).The more Vngf were injected, the more cpg-15 Mrna and NF-Κb Mrna were expressed in Vngf groups.Conclusions The Vngf could accelerate neural plasticity and restore neurofunctional defect through up-regulated the expression of cpg-15 and NF-Κb.
