Effect of the phagocytosis function on NR8383 ceil exposed to cigarette smoke extracts
10.3760/cma.j.issn.0254-5101.2011.03.015
- VernacularTitle:香烟烟气提取物对NR8383细胞吞噬功能的影响
- Author:
Wenjuan ZHANG
;
Xiujie PAN
;
Zhihua YANG
;
Wangshun HU
;
Jiangjin LIU
;
Feng CHEN
;
Maoxiang ZHU
- Publication Type:Journal Article
- Keywords:
Cigarette smoke extracts;
Phagocytosis;
NR8383 cells
- From:Chinese Journal of Microbiology and Immunology
2011;(3):261-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the phagocytosis function of cigarette smoke extracts (CSE)on the NR8383 cells. Methods The concentration of CSE and the optimal time was defined by cell counting kit-8 assay, Annexin V/PI cell apoptosis assay and CFSE cell proliferation assay. The cell was gained after exposed to the different concentration of CSE for 24 h and mixed with fluorescein-labeled Escherichia coli in 37℃ for 2 h. The fluorescence intensity was used to assay the phagocytosis function of NR8383 cells.Results The phagocytosis function of NR8383 cells may be changed by the concentration of CSE. In the concentration of 100 μg/ml, the phagocytosis function of NR8383 was enhanced 0.5 times than the normal cell when NR8383 cell was exposed to CSE, and the specific activity is the highest. When NR8383 cells were exposed to CSE and LPS, the phagocytosis function of NR8383 cells was enhanced 2 times than the normal cell. In the concentration of 200 μg/ml, the phagocytosis function of NR8383 cells was damaged, the rate of apoptosis is the 54. 1%. Conclusion Low concentration of CSE enhanced the phagocytosis function of NR8383 cells, but high concentration of CSE damaged the phagocytosis function of NR8383 cells. This study reveals a new role of CSE as an activator of macrophage function.
